We used the experimentally verified SADPr data and pS data derived from six studies[1-6] and PhosphositePlus database. We compared both datasets and found 3250 pSADPr peptides, 147,977 pS peptides, and 4270 SADPr peptides.
To prepare the benchmark data sets with high confidence for training and testing, we procedure established :
(1) In order to avoid over-estimation caused by similar protein sequences, Serine-modified peptides with >60% sequence identity clustered together and de-redundancy.
(2) The representative proteins in the dataset was randomly divided into two groups: 10/11 for cross-validation and the rest 1/11 for independent test.
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